Customer Inquiry

Date:
LABType Details
SSP Details
LABScreen Details
SmartType Details
AB Detection Details
TT Tray Details
Isolation Reagents Details
NXType Reagents Details
Software Details
SeCore Details
Equipment Details
 

PART 1 - Customer Information

Center ID:
Telephone No.:
Contact E-mail:
Invoice No.:
Center Name:
Contact/ Distributor:
Order No.:
 
 
 
 
PART 2 - Product Information

Product ID Lot# Batch/Dot#(Box#) Qnty Exp. Date Received Date Shipped Date Action
Catalog ID Quantity Serial Number Action
Product ID Version Build Action
PART 3 - Inquiry Description


PROBLEM CLARIFICATION for LABType:
 

Number of samples tested:
Number of samples with the described problem:
 
 

Is Problem:

 
 
 

PART 4 - TECHNICAL INFORMATION FOR LABTYPE PRODUCTS ONLY:
Please fill out this section if you are requesting technical support (PLEASE INCLUDE TEST DATE).
(Disclaimer: All OLI products are optimized for use with OLI reagents. We can not guarantee product performance if customer uses reagents other than OLI reagents, and/or if customer uses OLI products for purposes other than the ones stated in the OLI product inserts.)
STORAGE CONDITION OF REAGENTS PRIOR TO USE:
Stored at (˚C):
for month(s):
 
 
Have beads been refrozen:
Were beads protected from light exposure:
 
 

DNA AMPLIFICATION:
Extraction Method:
DNA Concentration (ng/ml):
 
 
DNA Purity (A260/A280):
 
 
 
 
Primer Set D-Mix used:
TAQ Polymerase:
 
 
OLI SSO PCR Program used:
PCR Machine:
 
 
Electrophoresis of PCR Product:
Can you send image:
 
 
Are excessive primer dimer bands at the bottom:
 
 
 
 
For A and B locus kits only, are the two specific primer bands visible (ave. 300 and 500 bps):
 
 
 
 

REAGENT PREPARATION AND HEATING DEVICE INFORMATION:
Protocol used:
Wash Technique used:
 
 
Was "wash step" carried out without interruption:
 
 
 
 
How much liquid left after flicking:
 
 
 
 
Heating Device used PCR Machine for Hybridization:
 
 
 
 
 
 
 
 
 
 
 
 
Set Temperature at (˚C):
 
 
 
 
SAPE and Bead Mixture kept in the dark:
 
 
 
 
SAPE Reconstitution Date:
Storage Condition:
 
 

DENATURATION/NEUTRALIZATION:
Color Change Noted: During Denaturation:
During Neutralization:
 
 

HYBRIDIZATION/LABELING:
Centrifuge:
Speed (g):
Time:
 
 
Centrifuge at Room Temp:
 
 
 
 
100X SAPE added to SAPE buffer during last wash::
 
 
 
 
Final Volume (µl):
 
 
 
 

DATA ACQUISITION:
Version of Luminex Data Acquisition Software Used:
If Other selected:
 
 
When Sample is ready:
 
 
 
 
 
for day(s) before testing:
 
 
 
Test Date:
Calibration Date:
 
 
d Cal (Δ) Temp:
 
 
 
 
Sheath PSI During Run:
Sheath PSI from Calibration:
 
 
Running Time of Sample:
Did it "sample empty":
 
 
Histogram Profile All:
 
 
 
 
Was needle height recently adjusted for the tray being used:
 
 
 
 

DATA ANALYSIS (please zip up and upload your session folder or output.csv file to this Form) :
NC Control Value:
 
 
 
 
PC Control Value:
Exon 2:
Exon 3:
 
 
Maximum overall false reaction setting:
Bead #'s Adjusted:
 
 


PROBLEM CLARIFICATION for SSP:
 

Number of samples tested:
Number of samples with the described problem:
 
 

Is Problem:
 
 
 

Typing Problem:
 
 
 
 
 
 
 
 

PART 4 - TECHNICAL INFORMATION FOR SSP PRODUCTS ONLY:
Please fill out this section if you are requesting technical support (PLEASE INCLUDE TEST DATE).
(Disclaimer: All OLI products are optimized for use with OLI reagents. We can not guarantee product performance if customer uses reagents other than OLI reagents, and/or if customer uses OLI products for purposes other than the ones stated in the OLI product inserts.)
STORAGE CONDITION OF REAGENTS PRIOR TO USE:
Stored at (˚C):
for month(s):
 
 
DNA INFORMATION:
Extraction method:
 
 
 
 
DNA Purity (A260/A280):
DNA Concentration (ng/µl):
 
 
Sample Information:
Sample Source
Please describe if Other:
 
 
Sample HLA Typing known?
If Yes, Please Note:
 
 
IMGT version used during analysis:
 
 
 
 

GEL INFORMATION:
Conc. Ethidium Bromide:
 
 
 
Running Buffer:
 
 

Agarose Manufacturer:
 
 
 
 
Agarose Concentration:
 
 
Gel System:
 
 
 
 
 
 
 
 
 
 
 

PCR INFORMATION:
PCR Machine:
 
 
 
 
PCR Program:
 
 
Plate Sealing Method:
 
 
 
 
PCR Ramp Rate
 
 
 
 
After tray is loaded:
 
Taq Manufacturer (Please indicate the Catalog ID of the TAQ next to the Manufacturer):
 
 
 
 
Storage Temp. of Tray/D-Mix (˚C):
 
 
 
 

PROBLEM CLARIFICATION for LABSCREEN:
 

Number of samples tested:
Number of samples with the described problem:
 
 

Is Problem:

 
 
 

PART 4 - TECHNICAL INFORMATION FOR LABSCREEN PRODUCTS ONLY:
Please fill out this section if you are requesting technical support (PLEASE INCLUDE TEST DATE).
(Disclaimer: All OLI products are optimized for use with OLI reagents. We can not guarantee product performance if customer uses reagents other than OLI reagents, and/or if customer uses OLI products for purposes other than the ones stated in the OLI product inserts.)
STORAGE CONDITION OF REAGENTS PRIOR TO USE:
Stored at (˚C):
for month(s):
 
 
Have beads been refrozen:
Were beads protected from light exposure:
 
 

SAMPLE INFORMATION
Sample Source:
 
 
 
 

Type:
Was the Serum spun down before processing
 
 

Drug Treatment:
 
 
 
 

Serum Treatment:
 
 
 

SAMPLE TESTING:
Protocol Used:
 
 
 
 

LS-AB2:
Lot:
Batch:
 
 

Exp. Date:
 
 
 
 

Washing Method Used:
 
 
 
 

NC Serum Used:

 
 
 

Configure:

Speed (g):
Time:
 
 

Final Volume of Sample:
 
 
 
 
DATA ACQUISITION
When Sample is Ready:
Temperature (˚C):
Days:

Version of Luminex Acquisition Software Used:
 
 
 
 

Test Date:
Calibration Date:
 
 

Running Time of Sample:
Did it "Sample Empty":
 
 
DATA ANALYSIS (please zip up and upload your session folder or output.csv file to this Form) :
Control Readings:
PC/NC Ratio:
PC (>500):
 
 

NC:
 
 
 
 

Default Settings for LABScreen:

Neg. (1):
Weak Pos.(2):
 
 

Pos.(4)
Strong Pos. (6):
 
 

Very Strong Pos. (8):
 
 
 
 

Advanced Settings for LABScreen Mixed:
Grey Area:
Positive:
 
 

PROBLEM CLARIFICATION for ANTIBODY DETECTION, ISOLATION REAGENTS & TISSUE TYPING PRODUCTS:
 

Number of samples tested:
Number of samples with the described problem:
 
 

Is Problem:
 
 
 

Average OD value of control wells (For LAT Products Only):
Blank:
Negative:
Postive:

PART 4 - TECHNICAL INFORMATION FOR ANTIBODY DETECTION, ISOLATION REAGENTS & TISSUE TYPING PRODUCTS ONLY:
Please fill out this section if you are requesting technical support (PLEASE INCLUDE TEST DATE).
(Disclaimer: All OLI products are optimized for use with OLI reagents. We can not guarantee product performance if customer uses reagents other than OLI reagents, and/or if customer uses OLI products for purposes other than the ones stated in the OLI product inserts.)
REAGENT INFORMATION:
Storage Temperature of tray (˚C):
Blood Draw Date:
 
 
Sample Source:
Type:
 
(For Antibody Detection) Serum Treatment:
 
 
 
Anticoagulant:
 
(For Tissue Typing) Tray Quality:
 
 
 
 
(Isolation Reagents) Reagent Quality:
 
 
 
 

TYPING TRAY USED: (FOR ISOLATION REAGENTS)
Tray ID:
 
 
 
Lot:
Batch:
COMPLEMENT
Cat ID:
 
 
 
Lot:
Batch:
STOPPING REAGENT
Cat ID:
 
 
 
Lot:
Batch:
 
 
Cat ID:
 
 
 
Lot:
Batch:
ISOLATION METHOD
Cat ID:
 
 
 
Lot:
Batch:
 
 
Cat ID:
 
 
 
Lot:
Batch:
CELL PREPARATION QUALITY (FOR TISSUE TYPING & ISOLATION REAGENTS)
Cell Yield (cells/ml):
Cell Viability(% of cell death)
 

 
PART 4 - TECHNICAL INFORMATION FOR SOFTWARE PRODUCTS ONLY:
Please fill out this section if you are requesting technical support (PLEASE INCLUDE TEST DATE).
(Disclaimer: All OLI products are optimized for use with OLI reagents. We can not guarantee product performance if customer uses reagents other than OLI reagents, and/or if customer uses OLI products for purposes other than the ones stated in the OLI product inserts.)
EXPLANATION OF ANALYSIS:
 
ERROR MESSAGE
 

PROBLEM CLARIFICATION for SECORE PRODUCTS:
 

Number of samples tested:
Number of samples with the described problem:
 
 

Is Problem:
 
 
 

 
 
 

 
 
 
 

Please describe if Other
 

PART 4 - TECHNICAL INFORMATION FOR SECORE PRODUCTS ONLY:
Please fill out this section if you are requesting technical support (PLEASE INCLUDE TEST DATE).
(Disclaimer: All OLI products are optimized for use with OLI reagents. We can not guarantee product performance if customer uses reagents other than OLI reagents, and/or if customer uses OLI products for purposes other than the ones stated in the OLI product inserts.)
DNA INFORMATION & AMPLIFICATION:
Sample Information:
Sample Source
Please describe if Other:
 
 
Sample HLA Typing known?
If Yes, Please Note:
If Yes, indicate typing method used:
IMGT version used during analysis:
 
 
 
 
Extraction Method:
 
 
 
 

DNA Purity (A260/A280):
DNA Concentration (ng/µl):
 
 
PCR Machine Used
Please describe if Other:
 
 
Plastics Used (Vendor)
Please describe if Other:
 
 
Was a post-PCR gel checked conducted?
 
 
 
 

EXOSAP-IT CLEAN-UP
ExoSAP-IT added to
Duration of vortex (please describe):
 
 
Centrifuge prior to PCR Machine?
 
 
 
 

SEQUENCING REACTION SETUP INFORMATION
Reaction setup on ice?
 
 
 
 
Plastics Used (Vendor)
Please describe if Other:
 
 
PCR Machine's block hot-started?
 
 
 
 
PCR Machine Used
Please describe if Other:
 
 

SEQUENCING REACTION CLEAN-UP
Method Used
 
 
 
 
Percentage of Stock EtOH
Please describe if Other:
 
 
Percentage of EtOH used for Wash
Please describe if Other:
 
 
Frequency of which EtOH used for wash is made
Please describe if Other:
 
 

CAPILLARY ELECTROPHORESIS
CE Instrument Utilized
Polymer Utilized
 
 
Duration of Polymer on Instrument (in days):
Duration of Anode and Cathode Buffer on Instrument (in days):
 
 
Array Length Utilized
Approximate Number of Injections on Current Array (if known)
 
 
Run Module Utilized
 
 
 
 


 
 

PART 4 - TECHNICAL INFORMATION FOR EQUIPMENT PRODUCTS ONLY:
Please fill out this section if you are requesting technical support (PLEASE INCLUDE TEST DATE).
(Disclaimer: All OLI products are optimized for use with OLI reagents. We can not guarantee product performance if customer uses reagents other than OLI reagents, and/or if customer uses OLI products for purposes other than the ones stated in the OLI product inserts.)
EQUIPMENT

WEEE Number:
 
 
 

RELEVANT AFFECTED COMPONENTS
Part Number Serial Number Action

EQUIPMENT COMMENTS

PROBLEM CLARIFICATION for SMARTTYPE PRODUCTS:
 

Number of samples tested:
Number of samples with the described problem:
 
 

Is Problem:

 
 
 

Typing Problem:
 
 
 

Test Info:
Negative Control:
Was Multichannel used?
 
 

PART 4 - TECHNICAL INFORMATION FOR SMARTTYPE PRODUCTS ONLY:
Please fill out this section if you are requesting technical support (PLEASE INCLUDE TEST DATE).
(Disclaimer: All OLI products are optimized for use with OLI reagents. We can not guarantee product performance if customer uses reagents other than OLI reagents, and/or if customer uses OLI products for purposes other than the ones stated in the OLI product inserts.)
STORAGE CONDITION OF REAGENTS PRIOR TO USE:

Stored at (˚C):
for month(s):
 
 
DNA INFORMATION:
Extraction Method:
Please describe if Other:
 
 

Sample Source:
Please describe if Other:
 
 

Sample HLA Typing known:
Please indicate typing method used:
 
 

DNA Concentration (ng/ml):
DNA Purity (A260/A280):
 
 

IMGT database version used during analysis:
Please provide attachments of all applicable ab1 files.
 
 
 

INSTRUMENT INFORMATION:
qPCR Instrument:
Please describe if Other:
 
 

STORAGE TEMPERATURE:

Storage temp of Master Mix after thaw (˚C):
 
Storage temp of Trays after opening kit (˚C):
 


PROBLEM CLARIFICATION for NXTYPE PRODUCTS:
 

Number of samples tested:
Number of samples with the described problem:
 
 

Is Problem:

 
 
 


PART 4 - TECHNICAL INFORMATION FOR NXTYPE REAGENTS PRODUCTS ONLY:
Please fill out this section if you are requesting technical support (PLEASE INCLUDE TEST DATE).
(Disclaimer: All OLI products are optimized for use with OLI reagents. We can not guarantee product performance if customer uses reagents other than OLI reagents, and/or if customer uses OLI products for purposes other than the ones stated in the OLI product inserts.)
DNA INFORMATION:

Sample Source:
Please describe if Other:
 
 

DNA Concentration (ng/ml):
DNA Purity (A260/A280):
 
 

PCR Machine used:
Please describe if Other:
 
 

If multiple PCR Machines used, please indicate model & step user for ALL steps:
 

Amplification Quantification method:
Please describe if Other:
 
 

Library Quantification method:
Please describe if Other:
 
 

Please describe any additional modification to the standard NXType Library preparation:
 

Please list ALL instances of stopping points with a duration >24h, along with storage:
 

IMPORTANT! In a single ZIP file, provide copies of:
- Ion Torrent Run Summary Report (PDF)
- Completed NXType Calculation Tool spreadsheet (Excel, all workbooks)
- Library Quantification Report/Export
- FASTQ/BAM/UBAM file (if sample specific)
 


Please Attach all Test Data:
(If there is more than one file please zip the files and only attach the zipped file.)
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